GENERAL INSTRUCTIONS
1. Eligibility: B. Sc. with Microbiology as principal subject and performance at entrance
examination. Students from Universities other than Univ. of Pune, must have
Microbiology as one of the subjects at third year B. Sc.
2. A full Master’s degree course in Sciences would be of 100 credits, where one credit
course of theory will be of one clock hour per week, running for 15 weeks and one credit
for practical course will consist of 15 clock hours of laboratory exercises.
3. For M.Sc. in Microbiology a student should take admission in the Microbiology
Department and complete at least 75% courses identified in the syllabus structure of
Microbiology. If students so desires remaining 25% courses can be chosen from courses
offered by other Departments with credit structure.
4. Course No. are designed to indicate the subject, semester, course serial number, credits
assigned and the nature as theory or practicals.
· MB indicates the subject, Microbiology
· 1st digit indicates the semester
· Last digit indicates the credits assigned to the course.
· Middle number indicates the serial number. The serial number beginning with zero
signifies that the course is a practical course.
eg. MB 1.1.1 means 1st semester, 1st paper with one credit
whereas, MB 1.01.5 means 1st semester, 1st practical course with five credits
5. Practical will be conducted throughout the academic year.
6. Some of the credit courses could consist of Seminars, Survey, field work, Special
laboratory training in Research Laboratories etc.
7. The in semester and end semester examinations will be of 50% marks each. This will
ensure that the students work regularly through the semester.
8. Question paper for each theory course will include at least one problem based on
research reports (Mathematical / Data Interpretation / Comment type) related to
concerned course.
Each course will be evaluated for 25 marks per credit of which 50 % will be based on
continuous / internal evaluation.
Credit Continuous/Internal
Assessment Marks
End Assessment
Marks
1 12 13
2 25 25
3 37 38
4 50 50
5 62 63
9. Results at the end of the semester will be declared using a grade point system.
10. The formula for GPA will be based on weighted average. The final GPA will not be
printed unless a student passed courses equivalent to minimum 100 credit hours. Total
ctredit hours means sum of credit hours of the courses which a student has passed.
Page 3 of 20
11. A seven point grade system (guided by the Government of Maharashtra Resolution No.
UGC-1298/[4619]/UNI. 4 dt December 11, 1999 and the University regulations) will be
followed. The corresponding grade table is as indicated
Explanation of Grades and Grade Point Average (GPA)
Marks Obtained Grade Grade Points
100-75 O Outstanding 06
74-65 A Very Good 05
64-55 B Good 04
54-50 C Average 03
49-45 D Satisfactory 02
44-40 E Pass 01
39 and less F Fail 00
Final Grade Points
Grade Points Final Grade
05.00-6.00 O
04.50-4.99 A
03.50-4.49 B
02.50-3.49 C
01.50-2.49 D
00.50-1.49 E
00.00-0.49 F
Common formula for GPA:
GPA (Grade Point Average) = Total of (Grade Points Earned X Credit hours for each course)
(Total Credit Hours)
‘B’ Grade is equivalent t at least 55% of the marks as per GR No. UGC-
1298/[4619]/UNI. 4 dt December 11, 1999
12. If the GPA is higher than the indicated upper limit in the third decimal digit, then the
student be awarded higher final grade (eg a student getting GPA of 4.492 may be awarded
‘A’.
13. There will be only final compilation and moderation at CGPA (Final) level done at the
Department. While declaring the result, the existing relevant ordinances are applicable.
There is also a provision for verification and revaluation. In case of versification the
existing rules will be applicable. The revaluation result will be adopted if there is a
change of at least 10% marks and in the grade of the course.
14. For grade improvement minimum 30 credit courses should be taken by the student. These
courses will be from the parent Department.
15. The above circular supersedes all previous circulars on the credit system being operated at
Department of Microbiology, University of Pune.
Page 4 of 20
M. Sc. (Microbiology) Curriculum
Semester I:
Course no. Title Total credits
MB 1.1.2 Systematics of Bacteria 2
MB1.2.1 Advanced Techniques in Taxonomy 1
MB 1.3.2 Basic Biochemistry 2
MB 1.4.1 Protein Chemistry 1
MB 1.5.1 Carbohydrate Chemistry 1
MB 1.6.1 Nucleic Acid Chemistry 1
MB 1.7.1 Lipid Chemistry 1
MB 1.8.1 Vitamin Chemistry 1
MB 1.9.2 Biology of Yeasts and Moulds 2
MB 1.10.1 Ultra structure of Cell Membrane 1
MB 1.11.1 Cell wall of Bacteria and Fungi 1
MB 1.12 .1 Microscopy 1
MB 1.13.2 Biochemical and Molecular Biology Techniques 2
MB 1.14.2 Biostatistics 2
Theory credits 19
Practicals
MB 1.01.5 Studies on Extremophiles 3
MB 1.02.5 Biochemical techniques 3
Practical credits 6
Total Credits 25
Page 5 of 20
Semester II:
Course no. Title Total credits
MB 2.1.2 General Virology 2
MB 2.2.1 Animal Virology 1
MB 2.3.1 Plant Virology 1
MB 2.4.1 Molecular Biology of Bacteriophages 1
MB 2.5.2 Bioenergetics 2
MB 2.6.2 Enzymology 2
MB 2.7.1 Photosynthesis 1
MB 2.8.1 Respiration: Aerobic, Anaerobic 1
MB 2.9.2 Environmental Biology 2
MB 2.10.2 Mathematics for Biologists 2
MB 2.11.2 Systematics of yeasts and molds 2
MB 2.12.1 Bioinformatics 1
MB 2.13.1 Biophysical techniques 1
MB 2.14.1 Radioisotopes in Biology 1
Theory credits 20
MB 2.01.5 Experimental Enzymology 3
MB 2.02.5 Ecology and Environmental Biology 3
Practical credits 6
Total Credits 26
Page 6 of 20
Department of Microbiology
University of Pune
Revised Syllabus for M. Sc. I Credit Course – w. e. f. July 2008
Course no. Title Description Total
credits
References
M.Sc. I Sem I Theory
MB 1.1.2 Systematics of
Bacteria
Species concept, Biological Nomenclature, theories
of biological classification, structural and
biochemical systematics, numerical taxonomy,
chemotaxonomy.
Biodiversity- characterization, generation
maintenance and loss; Magnitude and distribution of
Biodiversity, economic value, wild life biology,
conservation strategies, cryopreservation
2
Taxonomy
1. Barnett, H. L. and Hunter, B. B. 1960. Illustrated
Genera of Imperfect Fungi. Burgess Publishing Co.,
Minnesota.
2. Breed and Buchanan. Bergey’s Manual of
Determinative Bacteriology. 8th Edition, 1974.
3. Breed and Buchanan. Bergey’s Manual of
Determinative Bacteriology. 9th Edition, 1982.
4. Breed and Buchanan. Bergey’s Manual of Systematic
Bacteriology. 2nd Edition, (Volumes. 1 – 5) (2001 –
2003).
5. Lodder J. (1974). The Yeasts: A Taxonomic Study,
North Holland Publishing Co. Amsterdam.
6. Sykes, G. and F. A. Skinner (Eds). Actinomycetales:
Characteristics and Practical Importance. Society for
Applied Bacteriology Symposium Series No. 2,
Academic Press. 1973.
Diversity
1. Amann R. Ludwig W. and Schleifer K. (1995).
Phylogenetic Identification and In situ detection of
Individual Microbial Cells Without Cultivation,
Microbiological Reviews 59, 143-169.
Page 7 of 20
Course no. Title Description Total
credits
References
2. Cook T. (2002) Microbial Biodiversity: Saving Bacteria
to save ourselves, Harvard Science Review, 26-28.
3. Hugenholtz P. (2002) Exploring Prokaryotic Diversity
in the Genomic Era, Genome Biology, 3(2), 0003.1-
0003.8.
4. Keller M. and Zengler K. (2004) Tapping in to
Microbial Diversity. Nature Reviews 2, 141-150.
5. Pace N. (1997) A Molecular View of Microbial
Diversity and the Biosphere, Science, 276, 734-740.
6. Woese C. (1987), Bacterial Evolution. Microbiological
Reviews, 221-271.
MB1.2.1 Advanced
Techniques in
Taxonomy
molecular systematics, DNA fingerprinting, Gene
sequencing methods: 16S rDNA sequencing,
Multilocus sequence typing (MLST),
pyrosequencing, Sequencing by ligation, SOLiD
sequencing etc
1
MB 1.3.2 Basic Biochemistry 1. Bonds: ionic bonding, Ion-dipole. covalent, Hbonds,
Van der Wall’s interaction, Hydrophobic
and hydrophilic interactions.
2. Important biochemical reactions in metabolism
e.g. Substitution, Addition, Elimination,
Rearrangement, Oxidation, Reduction, Ring
closure, Transamination, Isomerisation,
Epimerization, Adenylation and Phosphorylation
Methylation, Hydrolysis, Dehydrase reaction,
Tautomerisation, etc.
3. Stereochemistry: Three-dimensional shapes of
molecules, conformation and configuration,
structure and biological activity relationship.
4. Concept of weak and strong acids and bases, pH,
Henderson-Hasselbalch equation, concept of
buffer, strength of buffer, range of buffer,
2 Chemical Composition of Living Systems
1. Berg Jeremy, Tymoczko John, Stryer Lubert (2001)
Biochemistry. 6th Edition, W. H. Freeman, New York.
2. Conn Eric, Stumpf Paul K., Bruuening George, Doi Roy
H., (1987) Outlines of Biochemistry. 5th Edition , John
Wieley and Sons, New Delhi.
3. Dawes Edwin A. (1972). Quantitative Problems in
Biochemistry, Churchill Livingston, Edimberg.
4. Laskin A. I. and Lechevalier H. A. (1977), CRC
Handbook of Microbiology, Vol. 1, Bacteria, CRC Press
Ohio.
5. Metzler David E. (2001) Biochemistry: The Chemical
Reactions of Living Cells, Volume 1 & 2, Academic
Press California.
6. Nelson D. L. and Cox M. M. (2002) Lehninger’s
Principles of Biochemistry, Mac Millan Worth Pub. Co.
Page 8 of 20
Course no. Title Description Total
credits
References
important biological buffers. New Delhi
MB 1.4.1 Protein Chemistry Structural features of amino acids, classification of
amino acids, amino acids as buffers, chemical
reactions of amino acids, peptide linkage: partial
double bond nature, determination of primary
structure of polypeptide (N-terminal, C-terminal
determination, method of sequencing of peptides),
structural classification of proteins, primary,
secondary, tertiary, quaternary structures of proteins.
1 7. Peberdy John F. (1980), Developmental Microbiology,
Blackie, London.
8. Segel Irvin H. (1997). Biochemical Calculations. 2nd
Ed. John Wiley and Sons, New York.
9. White Abraham, Handler Philip, Smith Emil, Hill
Rober, Lehman J. (1983) Principles of Biochemistry,
Edition 6, Tata Mc-Graw Hill Companies, Inc.
10. White David (2000) Physiology and Biochemistry of
Prokaryotes. 2nd Ed. Oxford University Press, New
York.
MB 1.5.1 Carbohydrate
Chemistry
Mono, di, oligosaccharides and polysaccharides,
with examples, asymmetric centre in sugars, Dseries,
L-series, dextro, leavo-rotatory, reducing and
non-reducing sugars, sugar anomers, sugar epimers,
sugar derivatives such as sugar alcohols, amino
sugars, sugar acids, deoxy sugars.
1
Introduction to Bioorganic Chemistry
1. Clayden, Greeves, Warren and Wothers, Organic
Chemistry, Oxford Press
Jerry March, Advanced Organic Chemistry, John Wiley
MB 1.6.1 Nucleic Acid
Chemistry
Structure of bases, nucleosides, nucleotides,
phospho-diester linkages, 5’ phosphate, 3’hydroxyl
polarity of nucleic acids, tautomeric forms of bases
and their implication in pairing of bases, structure of
DNA (A, B and Z forms), Tm value, detailed
structure of t-RNA, r-RNA, and m-RNA.
1
Page 9 of 20
Course no. Title Description Total
credits
References
MB 1.7.1 Lipid Chemistry Classification of lipids according to chemical
structure, fatty acids, saturated, unsaturated,
branched, nomenclature, system structure and
function of triglycerides, phospholipids,
sphingolipids, terpenes, prostaglandins, waxes,
steroids, detection and estimation of lipids
1
MB 1.8.1 Vitamin Chemistry Water and fat soluble vitamins: structure and
function of Thiamine, Riboflavin, Nicotinic acid,
Pantothenic acid, Pyridoxine, Biotin, Folic acid,
Lipoic acid, Glutathione, Vitamin B12, Ascorbic
acid. Structure and function of fat soluble vitamins
as vitamins A, D, E and K
1
MB 1.9.2 Biology of Yeasts
and Moulds
The vegetative cells of fungi, Growth and
development of the vegetative cell, The life cycle,
Asexual reproduction, Sexual reproduction,
Physiology of fungi, Economic importance of fungi,
Main groups of fungi
2 1. The Fungi Ed. Ainsworth , I. (1965), II (1966), III
(1968), IVA (1973), IVB (1973), Academic Press.
2. Booth, C. (1971) in Methods in Microbiology vol. 4,
ed. By J. R. Norris et al. , Academic Press, London.
3. Beech, F. W. and R. R. Davenport (1971) Isolation,
Purification and Maintenance of Yeasts, in Methods in
Microbiology vol. 4, ed. By J. R. Norris et al. ,
Academic Press, London, pp 153-182.
4. Rose, A. H. and J. S. Harrison ed. (1969) The Yeasts,
Vols. 1, 2, & 3. Academic Press, London.
MB 1.10.1 Ultra structure of
Cell Membrane
Ultrastructure and Organization of Eukaryotic
Cell
Structural organization of: Cytoskeleton (structural
proteins – microfilaments, actins, etc.); nucleus,
Mitochondria and chloroplasts and their genetic
organization, Endoplasmic Reticulum, Golgi
apparatus, Protein trafficking; Events in cell cycle,
Regulation of cell cycle
Membrane Transport:
1 Ultrastructure and Organization of Eukaryotic Cell
1. Alberts Bruce (1985) Molecular Biology of Cell.
Garland Pub.
2. Conn Eric, Stumpf Paul K., Bruuening George, Doi Roy
H., (1987) Outlines of Biochemistry Edition , John
Wieley and Sons, New Delhi.
3. De Robertis E. D. P. and De Robertis E. M. F. (1987),
Cellular and Molecular Biology Lea and Febiger,
Philadelphia.
Page 10 of 20
Course no. Title Description Total
credits
References
The composition and architecture of
membranes,Membrane dynamics, Solute transport
across membranes: Passive diffusion, active
transport using P and F type ATPases, Ion mediated
transport, transport of ions across membranes (ion
pumps), Biochemical shuttles across mitochondrial
membranes, Model membranes; Liposomes
4. Schlegel Hans G. (1995) General Microbiology, Edition
7, CUP, Cambridge.
5. Stanier R. Y., Adelberg E. A., Ingraham J. L., (1976)
General Microbiology, 4th edition, Mac Millan Press,
London.
6. Stephen W. Paddock, Confocal Microscopy, from
Methods and Protocols Vol. 122, Methods in Molecular
Biology, Humana Press, Press Inc., Totowa, NJ
Membrane Transport:
1. Nelson D. L. and Cox M. M. (2005) Lehninger’s
Principles of Biochemistry, Fourth edition, W. H.
Freeman & Co. New York.
2. Voet Donald and Voet Judith G. (1995) Biochemistry,
2nd Ed.. John Wiley and sons New York.
3. White Abraham, Handler Philip, Smith Emil, Hill
Rober, Lehman J. (1983) Principles of Biochemistry,
Edition 6, Tata Mc-Graw Hill Companies, Inc.
4. White David (2000) Physiology and Biochemistry of
Prokaryotes. 2nd Ed. Oxford University Press, New
York.
5. Zubay Geoffrey (1998) Biochemistry, 4th Ed., W. C.
Brown, New York.
6. Berg Jeremy, Tymoczko John, Stryer Lubert (2001)
Biochemistry 4th Ed, W. H. Freeman, New York.
7. Moat Albert G. and Foster John W. (1988) Microbial
Physiology 2nd Ed. John Wiley and Sons New York.
MB 1.11.1 Cell wall of
Bacteria and Fungi
Definition, important chemical features of the cell
wall, structure, function and assembly in bacteria and
archae, typical model of cell wall of bacteria and
archae, chemical differentiation of fungal cell wall
1 1. Ruiz-Herrera J, (1991) Fungal cell walls. CRC Press
ISBN:0849366720
2. Guntram Seltmann, Otto Holst (2001) Bacterial cell
walls. Springer ISBN:3540426086
MB 1.13.2 Biochemical and
Molecular Biology
Techniques
A. Principles and applications of gel filtration, ion
exchange and affinity chromatography, thin layer
and gas chromatography, high pressure liquid
chromatography (HPLC), electrophoresis and
electrofocussing, ultra centrifugation (velocity
2 Instrumentation
1. Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006)
Biochemistry. 6th Edition. Freeman, New York.
2. Cotterill, R. M. J. (2002) Biophysics: An Introduction.
John Wiley & Sons, England.
Page 11 of 20
Course no. Title Description Total
credits
References
and buoyant density
B. Principles and techniques of nucleic acid
hybridization and Cot curves; Sequencing of
Proteins and nucleic acids ; Southern, Northern
and South-Western blotting techniques ;
Polymerase chain reaction; Methods for
measuring nucleic acid & protein interactions
3. Garrett, R. H. and Grisham, C. M. (2004) Biochemistry.
3rd Ed. Brooks/Cole, Publishing Company, California.
4. Mount, D. W. (2001) Bioinformatics: sequence and
genome analysis. Cold Spring Harbor Laboratory Press,
New York.
5. Nölting, B. (2006) Methods in modern biophysics.
Second Edition. Springer, Germany.
6. Pattabhi, V. and Gautham, N. (2002) Biophysics.
Kluwer Academic Publishers, New York & Narosa
Publishing House, Delhi.
7. Wilson Keith and Walker John (2005) Principles and
Techniques of Biochemistry and Molecular Biology, 6th
Ed. Cambridge University Press, New York.
Molecular Biophysics
1. Daune M. & W. J. Duffin (1999) Molecular Biophysics:
Structures in Motion, Oxford University Press.
2. Nalting B. & B. Nalting (2003) Methods in Modern
Biophysics Springer Verlag
3. Voit E. O. (2000) Computational Analysis of
Biochemical Systems Cambridge University Press.
Other books:
1. Narayanan, P. (2000) Essentials of Biophysics. New
Age International Publication, New Delhi
2. Stephenson, F. H. (2003) Calculations in molecular
biology and biotechnology: A guide to mathematics in
the laboratory. Academic Press, Elsevier Science,
London. (For numerical problems in instrumentation)
MB 1.14.2 Biostatistics a. Quantitative methods in biology, sampling
methods, scales and variables, data organization,
tabulation, graphical representation
b. Concepts, examples and problems for each of the
following:
i. Descriptive statistics: Frequency and
2 1. Brown D. and Rothery P. 1993. Models in Ecology,
Wiley.
2. Cochran W. G. – Sampling Techniques, Wiley estern
Ltd, New Delhi.
3. Felller W. Introduction to probability theory and its
applications, Asia Publishing House, Mumbai.
Page 12 of 20
Course no. Title Description Total
credits
References
probability distributions, graphical
representation of distributions, measures of
central tendency, measures of dispersion,
skew-ness, kurtosis. Introduction to Normal,
Binomial and Poisson distributions and their
applications. Distribution of sample means,
standard error and confidence interval.
ii. Regression and correlation, curve fitting and
choice of models.
iii. Introduction to multivariate analysis: multiple
regressions, ordination, principal component
analysis.
iv. Survey design
v. Factorial design, ANOVA and F test.
vi. Probability: Laws of probability, independence
and randomness.
vii. Hypothesis testing: Comparison of two sample
means: t-tests, non-parametric tests. The
concepts of null hypothesis,
significance level, type I and type II errors, one
tailed and two tailed tests.
Categorical data and proportion data: Chi square test
and test for goodness of fit.
1. Glover T. and Mitchell K. 2002. An introduction to
Biostatistics. McGraw-Hill , N.Y.
2. Goon, Gupta and Dasgupta- Fundamentals of statistics.
World Press, Kolkota.
3. Irfan Ali Khan and Atiya Khanum, Fundamentals of
Biostatistics. 2nd Ed. Ukaaz Publications, Hyderabad.
4. Montgomery D. C. Design and analysis of experiments,
John Wiley and Sons.
5. Murthy M.N. Sampling methods, Indian Statistical
Institute, Kolkota.
6. Wayne Daniel 2007. Biostatistics, a foundation for
analysis in the health Sciences, Edn. 7, Wiley-Indian
Edn.
Theory Credits 19
M.Sc. I Sem I Practicals
MB 1.01.5 Studies on
Extremophiles
1. Isolation and characterization of anaerobic
microorganisms
2. Isolation and characterization of thermophilic
microorganisms
3. Isolation and characterization of Psychrophilic
microorganisms
4. Isolation and characterization of Alkalophilic
microorganisms
5. Isolation and characterization of Acidophilic
3
Page 13 of 20
Course no. Title Description Total
credits
References
microorganisms
6. Isolation and characterization of Halopphilic
microorganisms
MB 1.02.5 Biochemical
techniques
A) Basic Biochemistry
1. Buffer: Determination of pKa of a monoprotic
weak organic acid; Preparation of buffers using
KH2PO4 and K2HPO4, acetic acid and sodium
acetate, K2HPO4 and H3PO4
2. Colorimetry and spectrophotometry: Estimation
of sugar and total carbohydrate, estimation of
protein and nucleic acids ( DNSA, phenolsulphuric
acid method, Lowry, Bradford and
UV Spectrophotometry, Diphenyl amine and
orcinol method)
3. Determination of saponification value and iodine
number of fat
4. Chromatography: Separation of sugar and
amino acids by paper and thin layer
chromatography
5. Electrophoresis: Agarose gel electrophoresis,
PAGE and SDS-PAGE of proteins
6. Molecular weight determination by Moecular
sieve chromatography
B) Cell Disruption and separation of cell
components using Sucrose/ Ficoll Density
Gradient( yeast)
3 1. Wilson Keith and Walker John (2005) Principles and
Techniques of Biochemistry and Molecular Biology, 6th
Ed. Cambridge University Press, New York.
Practical Credits 6
Total Credits 25
Page 14 of 20
Course no. Title Description Total
credits
References
M.Sc. I Sem II Theory
MB 2.1.2 General Virology 1) Distinctive properties of viruses: size, acellular
(non cellular) organization, nature of viral
genome, etc.
2) Morphology and ultra structure of viruses:
Capsids; icosahedral, helical, envelope,
glycoprotein, matrix proteins and lipids, Viral
genome (double stranded viral DNA / RNA,
single stranded viral DNA / RNA, etc); virus
related agents (viroids, prions).
3) Cultivation of viruses: Growth of viruses in
embryonated egg, in experimental animals and
in cell cultures-primary and secondary cell lines,
suspension cell cultures and monolayer cell
cultures.
4) Assay of viruses: Physical and chemical
methods of assay, (protein, nucleic acid,
radioactivity tracers, electron microscopy, etc);
Infectivity assay of animal viruses (plaque
method, pock counting, end point method) and
infectivity assay of plant viruses.
5) Morphology and ultra-structure of
bacteriophages, one step growth curve(latent
period, Eclipse period, burst size), life cycle and
other details with special reference to T (odd and
even).
2 7. Straus J. H. and Straus E.S. (1998) Evolution of RNA
Viruses Ann. Rev. Microbiol. 42: 657 – 83
8. Luria S. E. et.al. (1978) General virology, 3rd Ed, New
York. John Wiley and Sons.
9. Fields B.N.; Knipe D. M. Chanock R.M. Hirsch M. J. (Eds)
Fields Virology, 2nd Ed. New York, Raven Press. (1996)
MB 2.2.1 Animal Virology 1) Classification and nomenclature of viruses: ICTV
recommendations
2) Life cycles and other details : DNA viruses with
special reference to herpes, pox, adeno, SV40;
RNA viruses with special reference to measles,
rabies, polio, influenza, retroviruses; Oncoviruses
and Lentiviruses (HIV).
3) Miscellaneous: Interferon, antiviral agents, slow
1 1. Stephens B. and Compons R. W. (1998) Assembly of
animal viruses at the cellular membrane Ann. Rev.
Microbiol.42:489-519
2. Reisner D. & Gross H.J. (1985) Viroids Ann. Rev.
Biochem.54:531-64
3. Prusiner S. B. (1995) The Prion Diseases, Scientific
American (1):48-57
4. Sherkar A. H. & Marion P.L. (1991) Hepo DNA viruses and
Page 15 of 20
Course no. Title Description Total
credits
References
and persistent viruses; Mechanism of virus host
interaction / Mechanism of virus persistence.
Hepatocellular Carcinomas. Ann. Rev. Microbiol.45:475-
508
5. Davis and Dulbacco Medical Microbiology
MB 2.3.1 Plant Virology 1) Classification and nomenclature.
2) Effects of viruses on plants: appearance of plants,
histology, physiology and cytology of plants.
3) Diagnostic techniques to detect viruses: In seeds,
seed stocks, and diseased plants (seed
morphology, seedling symptomatology, indicator
plants, serological methods, histochemical tests
and fluorescence microscopy.)
4) Behavior of viruses in plants: Early stages of
infection, biochemistry of virus replication,
cellular sites of virus replication and assembly
and accumulation of virus particles.
5) Transmission of plant viruses: With vectors
(insects, nematodes, fungi, etc.) without vectors
(contact, seed and pollens).
6) Prevention of crop losses due to virus infection:
virus free planting material, vector control,
disease forecasting.
7) Life cycle and other details of few plant viruses:
With special reference to TMV, Cauliflower
mosaic virus etc.
1 Gibbs Adrian & Bryan Harrison ( ) Plant Virology -The
Principles. Edward Arnold Press
MB 2.4.1 Molecular
Biology of
Bacteriophages
DNA replication in T phages, lambda phage, different
mutants in phages: rapid lysis mutants (rI, rII, rIII),
Conditional mutants e.g clear plaque mutants in l,
mechanism of packing DNA in phage heads,
Lambda- lytic cascade and lysogenic repression;
M13 replication.
1 1. Matthews R.E.F. (1985) Viral Taxonomy for the Non
virologists Ann. Rev. Microbiol. 39:451-74
MB 2.5.2 Bioenergetics Laws of thermodynamics, entropy, enthalpy, free
energy, free energy and equilibrium constant, Gibbs
free energy equation, determination of free energy of
hydrolytic and biological oxidation reduction
reactions, under standard and non-standard
2
Page 16 of 20
Course no. Title Description Total
credits
References
conditions, high energy compounds, coupled
reactions, determination of feasibility of reactions.
MB 2.6.2 Enzymology Purifications of enzyme, purification chart, kinetics of
single substrate enzyme catalyzed reaction. Kinetics
of reversible inhibitions enzyme catalyzed reactions,
King Altman approach to derive – two substrate
enzyme catalyzed reactions, types of two substrate
enzyme catalyzed reactions, concept of allosterism,
positive and negative co-operativity, models of
allosteric enzymes (Monad, Wyamann and Changuax
and Koshland, Nemethy and Filmer model), kinetics
of allosteric enzyme, Hill plot, examples of allosteric
enzymes and their significance in regulation.
2 1. Berg Jeremy, Tymoczko John, Stryer Lubert (2001)
Biochemistry 4th Ed, W. H. Freeman, New York.
2. Conn Eric, Stumpf Paul K., Bruuening George, Doi Roy H.,
(1987) Outlines of Biochemistry 5th Ed , John Wiley and
Sons, New Delhi.
3. Dawes Edwin A. (1972) Quantitative Problems in
Biochemistry, Churchill Livingston, Edinburgh.
4. Hall D. D. & Rao K. K. (1996) Photosynthesis 5th Ed.,
Cambridge University Press.
5. Mandelstam Joel and McQuillen Kenneth (1976)
Biochemistry of Bacterial Growth, Blackwell Scientific
Publication London.
6. Metzler David (2001) Biochemistry: The chemical Reactions
of Living Cells, Vol 1&2, Academic Press California.
7. Moat Albert G. & Foster John W. (1988) Microbial
Physiology 2nd Ed. John Wiley and Sons New York.
8. Nelson D. L. & Cox M. M. (2005) Lehninger’s Principles of
Biochemistry, 4th edition, W. H. Freeman & Co. NY
9. Palmer Trevor (2001) Enzymes: Biochemistry,
Biotechnology & Clinical chemistry, Horwood Pub. Co.,
England.
10. Segel Irvin H. (1997) Biochemical Calculations 2nd Ed.,
John Wiley and Sons, New York.
11. Voet Donald & Voet Judith G. (1995) Biochemistry, 2nd Ed..
John Wiley & sons New York.
12. White Abraham, Handler Philip, Smith Emil, Hill Rober,
Lehman J. (1983) Principles of Biochemistry, Edn 6, Tata
Mc-Graw Hill Companies, Inc.
13. White David (2000) Physiology and Biochemistry of
Prokaryotes. 2nd Ed. Oxford University Press, NY.
14. Zubay Geoffrey (1998) Biochemistry, 4th Ed., W.C. Brown,
New York.
Page 17 of 20
Course no. Title Description Total
credits
References
MB 2.7.1 Photosynthesis Energy consideration in photosynthesis, light and
dark reaction, electron carriers in photosynthesis,
Organization of photosystem I and II, cyclic and noncyclic
flow of electrons, Z scheme, Hill reaction,
photolysis of water. Bacterial photosynthesis: scope,
electron carriers, Photosynthetic reaction center,
cyclic flow of electrons, bacterial
photophosphorylation in various groups of
phototrophic bacteria, electron donors other than
water in anoxygenic photosynthetic bacteria.
1
MB 2.8.1 Respiration:
Aerobic,
Anaerobic
Aerobic Respiration:
Mitochondrial electron transport chain, structure and
function of ATPase (bacterial and mitochondrial),
generation and maintenance of proton motive force,
oxidative phosphorylation, inhibitors and un-couplers
of electron transport chain and oxidative
phosphorylation, Atkinson’s energy charge,
phosphorylation potential and its significance, Energy
generation in all groups of chemolithotrophs.
Anaerobic Respiration:
Concept of anaerobic respiration, oxidized sulfur
compounds, and nitrate as electron acceptor with
respect to electron transport chain and energy
generation, Biochemistry of methanogenes
1
MB 2.9.2 Environmental
Biology
A. Interactions between environment and biota ;
Concept of habitat and ecological niches;
Limiting factor ; Energy flow, food chain, food
web and tropic levels ; Ecological pyramids and
recycling, biotic community – concept, structure,
dominance, fluctuation and succession; N, P, C
and S cycles in nature.
2 1. Dash, M.C. (1993). Fundamentals of Ecology. Tata McGrw
Hill Publishing Hill Co. Ltd., New Delhi
2. Macan, T. T. (1974). Freshwater Ecology. Longman Group
Ltd., London
3. Meadows, P. S. and Campbell. (1978). An introduction to
Marine Science. Blackie and Sons Ltd., Glasgow.
4. Richards, B. N. (1987). Microbiology of Terrestrial
Page 18 of 20
Course no. Title Description Total
credits
References
B. Ecosystem dynamics and management; Stability
and complexity of ecosystems; Speciation and
extinctions; Environmental impact assessment,
Principles of conservation; Conservation
strategies; Sustainable development.
Ecosystems. Longman Scientific and Technical, N.Y.
5. Techobanoglous G. and F. L. Burton (1991). Wastewater
Engineering, Treatment, Disposal and Reuse. 3rd Ed.,
Metccalf and Eddy (Eds) Tata McGraw Hill Publishing Co.
Ltd., New Delhi.
MB 2.10.2 Mathematics for
Biology
Numbers, variables (independent, dependent and
continuous), Definition of Mathematical Function:
linear, geometric and exponential functions;
differentiation and integration of simple functions,
graphs, plots.etc.
2 Arya J.A and Lardner R.W. (1979) Mathematics for Biological
Sciences. Prentice Hall Inc.
MB 2.11.2 Systematics of
yeasts and molds
Taxonomy,( classification, nomenclature and
identification) and systematics of fungi
Classification of yeasts and Molds: Characteristics as
criteria for classification, , A system of yeast
classification, 18S rDNA nucleotide sequencing
based classification of fungi, A system of
classification of molds, Systematics of fungi
2 1. Ainsworth, G. C.(1971) Dictionary of Fungi, CMI, Surrey.
2. The Fungi Ed. Ainsworth , I. (1965), II (1966), III (1968),
IVA (1973), IVB (1973), Academic Press.
3. Barnett, J. A. , R. W. Payne, and D. Yarrow (1979) A guide
to identifying and classifying yeasts, Cambridge Univ. Press,
Cambridge.
4. Campbell, I. (1974) Methods of Numerical Taxonomy for
various genra of Yeasts, in Advances in Applied Microbiology,
ed. By D. Perlman, A.P., N.Y., 17: 135-156.
5. Hawksworth, D. L. (1974). Mycologist’s Handbook, CMI
MB 2.12.1 Bioinformatics
1. Databases
Biological resource databases – Examples and
application – sequence Analysis – protein and
nucleic acid.
2. Genomics and proteomics
Sequencing genomes – sequence assembly –
genome on the web – annotating and analyzing
genome sequences. proteomics – biochemical
pathway databases.
3. Sequence analysis
Pair wise sequence comparison. protein data
bank, Swiss-prot, Genebank – sequence queries
1 1. Baldi, P. and Brunak, S. (2001) Bioinformatics: The
machine learning approach. Bradford Book, MIT Press,
Cambridge.
2. Baxevanis, A. D. and Ouellette, B. F. F. (2001)
Bioinformatics: A practical guide to the analysis of genes
and proteins. 2nd Edition. John Wiley & Sons, New York.
3. Ewens Warren J. and Gregory R. Grant. (2004) Statistical
Methods in Bioinformatics, An Introduction, Springer, New
York.
4. Lacroix, Z. and Critchlow, T. (Eds.) 2003. Bioinformatics.
Managing Scientific Data. Morgan Kaufmann Publishers.
5. Misener, S. and Krawetz, S. A. (Eds.). 2000. Methods in
Molecular Biology, Volume 132. Bioinformatics: Methods
Page 19 of 20
Course no. Title Description Total
credits
References
against biological databases – BLAST and
FASTA – multifunctional tools for sequence
analysis. multiple sequence alignments,
phylogenetic alignment – profiles and motifs.
& Protocols. Humana Press, New Jersey.
6. Mount, D. W. (2001) Bioinformatics: sequence and genome
analysis. Cold Spring Harbor Laboratory Press, NY.
7. Zoe L. & Terence C. (2004) Bioinformatics: Managing
Scientific Data, Morgan Kaufmann Publishers, New Delhi.
MB 2.13.1 Biophysical
techniques
Principles of biophysical methods used for analysis of
biopolymer structure, X-ray diffraction, fluorescence,
UV, GRO, CO, Visible, NMR and ESR spectroscopy
; Hydrodynamic methods, Atomic absorption and
plasma emission spectroscopy.
1 Instrumentation
1. Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006)
Biochemistry. 6th Edition. Freeman, New York.
2. Cotterill, R. M. J. (2002) Biophysics: An Introduction. John
Wiley & Sons, England.
3. Drenth, J. (2007) Principles of protein X-ray
crystallography. 3rd Ed. Springer, Germany.
4. Garrett, R. H. and Grisham, C. M. (2004) Biochemistry.
3rd Ed. Brooks/Cole, Publishing Company, California.
5. Keeler, J. (2002) Understanding NMR Spectroscopy. John
Wiley & Sons, England.
6. Mount, D. W. (2001) Bioinformatics: sequence and
genome analysis. Cold Spring Harbor Laboratory Press,
New York.
7. Nölting, B. (2006) Methods in modern biophysics. Second
Edition. Springer, Germany.
8. Pattabhi, V. and Gautham, N. (2002) Biophysics. Kluwer
Academic Publishers, New York and Narosa Publishing
House, Delhi.
9. Wilson Keith and Walker John (2005) Principles and
Techniques of Biochemistry and Molecular Biology, 6th Ed.
Cambridge University Press, New York.
MB 2.14.1 Radioisotopes in
Biology
Principles and applications of tracer techniques in
biology ; Radiation dosimetry ; Radioactive isotopes
and half life of isotopes ; Effect of radiation on
biological system ; Autoradiography ; Cerenkoy
radiation ; Liquid spectrometry.
1 Molecular Biophysics
1. Cavanagh John et.al. (1995) Proteins NMR Spectroscopy:
Principles and Practice, Academic Press
2. Daune M. & W. J. Duffin (1999) Molecular Biophysics:
Structures in Motion, Oxford University Press.
3. Nalting B. & B. Nalting (2003) Methods in Modern
Biophysics Springer Verlag
Page 20 of 20
Course no. Title Description Total
credits
References
4. Voit E. O. (2000) Computational Analysis of Biochemical
Systems Cambridge University Press.
Other books:
1. Narayanan, P. (2000) Essentials of Biophysics. New Age
International Publication, New Delhi
Stephenson, F. H. (2003) Calculations in molecular biology and
biotechnology: A guide to mathematics in the laboratory.
Academic Press, Elsevier Science, London. (For numerical
problems in instrumentation)
Theory Credits 22
M.Sc. I Sem II Practicals
MB 2.01.5 Experimental
Enzymology
1. Purification of enzyme from natural source
2. Determination of Km and Vm values of
enzyme :e.g.Invertase,amylase
3
MB 2.02.5 Ecology and
Environmental
Biology
1. Isolation, of cellulose degraders
2. Isolation of chitinase degraders
3. Isolation of pesticide degraders.
4. Isolation of Aflatoxin producing organism
5. Detection of Aflatoxin in food / culture
3
Practical Credits 6
Total Credits 26
1. Eligibility: B. Sc. with Microbiology as principal subject and performance at entrance
examination. Students from Universities other than Univ. of Pune, must have
Microbiology as one of the subjects at third year B. Sc.
2. A full Master’s degree course in Sciences would be of 100 credits, where one credit
course of theory will be of one clock hour per week, running for 15 weeks and one credit
for practical course will consist of 15 clock hours of laboratory exercises.
3. For M.Sc. in Microbiology a student should take admission in the Microbiology
Department and complete at least 75% courses identified in the syllabus structure of
Microbiology. If students so desires remaining 25% courses can be chosen from courses
offered by other Departments with credit structure.
4. Course No. are designed to indicate the subject, semester, course serial number, credits
assigned and the nature as theory or practicals.
· MB indicates the subject, Microbiology
· 1st digit indicates the semester
· Last digit indicates the credits assigned to the course.
· Middle number indicates the serial number. The serial number beginning with zero
signifies that the course is a practical course.
eg. MB 1.1.1 means 1st semester, 1st paper with one credit
whereas, MB 1.01.5 means 1st semester, 1st practical course with five credits
5. Practical will be conducted throughout the academic year.
6. Some of the credit courses could consist of Seminars, Survey, field work, Special
laboratory training in Research Laboratories etc.
7. The in semester and end semester examinations will be of 50% marks each. This will
ensure that the students work regularly through the semester.
8. Question paper for each theory course will include at least one problem based on
research reports (Mathematical / Data Interpretation / Comment type) related to
concerned course.
Each course will be evaluated for 25 marks per credit of which 50 % will be based on
continuous / internal evaluation.
Credit Continuous/Internal
Assessment Marks
End Assessment
Marks
1 12 13
2 25 25
3 37 38
4 50 50
5 62 63
9. Results at the end of the semester will be declared using a grade point system.
10. The formula for GPA will be based on weighted average. The final GPA will not be
printed unless a student passed courses equivalent to minimum 100 credit hours. Total
ctredit hours means sum of credit hours of the courses which a student has passed.
Page 3 of 20
11. A seven point grade system (guided by the Government of Maharashtra Resolution No.
UGC-1298/[4619]/UNI. 4 dt December 11, 1999 and the University regulations) will be
followed. The corresponding grade table is as indicated
Explanation of Grades and Grade Point Average (GPA)
Marks Obtained Grade Grade Points
100-75 O Outstanding 06
74-65 A Very Good 05
64-55 B Good 04
54-50 C Average 03
49-45 D Satisfactory 02
44-40 E Pass 01
39 and less F Fail 00
Final Grade Points
Grade Points Final Grade
05.00-6.00 O
04.50-4.99 A
03.50-4.49 B
02.50-3.49 C
01.50-2.49 D
00.50-1.49 E
00.00-0.49 F
Common formula for GPA:
GPA (Grade Point Average) = Total of (Grade Points Earned X Credit hours for each course)
(Total Credit Hours)
‘B’ Grade is equivalent t at least 55% of the marks as per GR No. UGC-
1298/[4619]/UNI. 4 dt December 11, 1999
12. If the GPA is higher than the indicated upper limit in the third decimal digit, then the
student be awarded higher final grade (eg a student getting GPA of 4.492 may be awarded
‘A’.
13. There will be only final compilation and moderation at CGPA (Final) level done at the
Department. While declaring the result, the existing relevant ordinances are applicable.
There is also a provision for verification and revaluation. In case of versification the
existing rules will be applicable. The revaluation result will be adopted if there is a
change of at least 10% marks and in the grade of the course.
14. For grade improvement minimum 30 credit courses should be taken by the student. These
courses will be from the parent Department.
15. The above circular supersedes all previous circulars on the credit system being operated at
Department of Microbiology, University of Pune.
Page 4 of 20
M. Sc. (Microbiology) Curriculum
Semester I:
Course no. Title Total credits
MB 1.1.2 Systematics of Bacteria 2
MB1.2.1 Advanced Techniques in Taxonomy 1
MB 1.3.2 Basic Biochemistry 2
MB 1.4.1 Protein Chemistry 1
MB 1.5.1 Carbohydrate Chemistry 1
MB 1.6.1 Nucleic Acid Chemistry 1
MB 1.7.1 Lipid Chemistry 1
MB 1.8.1 Vitamin Chemistry 1
MB 1.9.2 Biology of Yeasts and Moulds 2
MB 1.10.1 Ultra structure of Cell Membrane 1
MB 1.11.1 Cell wall of Bacteria and Fungi 1
MB 1.12 .1 Microscopy 1
MB 1.13.2 Biochemical and Molecular Biology Techniques 2
MB 1.14.2 Biostatistics 2
Theory credits 19
Practicals
MB 1.01.5 Studies on Extremophiles 3
MB 1.02.5 Biochemical techniques 3
Practical credits 6
Total Credits 25
Page 5 of 20
Semester II:
Course no. Title Total credits
MB 2.1.2 General Virology 2
MB 2.2.1 Animal Virology 1
MB 2.3.1 Plant Virology 1
MB 2.4.1 Molecular Biology of Bacteriophages 1
MB 2.5.2 Bioenergetics 2
MB 2.6.2 Enzymology 2
MB 2.7.1 Photosynthesis 1
MB 2.8.1 Respiration: Aerobic, Anaerobic 1
MB 2.9.2 Environmental Biology 2
MB 2.10.2 Mathematics for Biologists 2
MB 2.11.2 Systematics of yeasts and molds 2
MB 2.12.1 Bioinformatics 1
MB 2.13.1 Biophysical techniques 1
MB 2.14.1 Radioisotopes in Biology 1
Theory credits 20
MB 2.01.5 Experimental Enzymology 3
MB 2.02.5 Ecology and Environmental Biology 3
Practical credits 6
Total Credits 26
Page 6 of 20
Department of Microbiology
University of Pune
Revised Syllabus for M. Sc. I Credit Course – w. e. f. July 2008
Course no. Title Description Total
credits
References
M.Sc. I Sem I Theory
MB 1.1.2 Systematics of
Bacteria
Species concept, Biological Nomenclature, theories
of biological classification, structural and
biochemical systematics, numerical taxonomy,
chemotaxonomy.
Biodiversity- characterization, generation
maintenance and loss; Magnitude and distribution of
Biodiversity, economic value, wild life biology,
conservation strategies, cryopreservation
2
Taxonomy
1. Barnett, H. L. and Hunter, B. B. 1960. Illustrated
Genera of Imperfect Fungi. Burgess Publishing Co.,
Minnesota.
2. Breed and Buchanan. Bergey’s Manual of
Determinative Bacteriology. 8th Edition, 1974.
3. Breed and Buchanan. Bergey’s Manual of
Determinative Bacteriology. 9th Edition, 1982.
4. Breed and Buchanan. Bergey’s Manual of Systematic
Bacteriology. 2nd Edition, (Volumes. 1 – 5) (2001 –
2003).
5. Lodder J. (1974). The Yeasts: A Taxonomic Study,
North Holland Publishing Co. Amsterdam.
6. Sykes, G. and F. A. Skinner (Eds). Actinomycetales:
Characteristics and Practical Importance. Society for
Applied Bacteriology Symposium Series No. 2,
Academic Press. 1973.
Diversity
1. Amann R. Ludwig W. and Schleifer K. (1995).
Phylogenetic Identification and In situ detection of
Individual Microbial Cells Without Cultivation,
Microbiological Reviews 59, 143-169.
Page 7 of 20
Course no. Title Description Total
credits
References
2. Cook T. (2002) Microbial Biodiversity: Saving Bacteria
to save ourselves, Harvard Science Review, 26-28.
3. Hugenholtz P. (2002) Exploring Prokaryotic Diversity
in the Genomic Era, Genome Biology, 3(2), 0003.1-
0003.8.
4. Keller M. and Zengler K. (2004) Tapping in to
Microbial Diversity. Nature Reviews 2, 141-150.
5. Pace N. (1997) A Molecular View of Microbial
Diversity and the Biosphere, Science, 276, 734-740.
6. Woese C. (1987), Bacterial Evolution. Microbiological
Reviews, 221-271.
MB1.2.1 Advanced
Techniques in
Taxonomy
molecular systematics, DNA fingerprinting, Gene
sequencing methods: 16S rDNA sequencing,
Multilocus sequence typing (MLST),
pyrosequencing, Sequencing by ligation, SOLiD
sequencing etc
1
MB 1.3.2 Basic Biochemistry 1. Bonds: ionic bonding, Ion-dipole. covalent, Hbonds,
Van der Wall’s interaction, Hydrophobic
and hydrophilic interactions.
2. Important biochemical reactions in metabolism
e.g. Substitution, Addition, Elimination,
Rearrangement, Oxidation, Reduction, Ring
closure, Transamination, Isomerisation,
Epimerization, Adenylation and Phosphorylation
Methylation, Hydrolysis, Dehydrase reaction,
Tautomerisation, etc.
3. Stereochemistry: Three-dimensional shapes of
molecules, conformation and configuration,
structure and biological activity relationship.
4. Concept of weak and strong acids and bases, pH,
Henderson-Hasselbalch equation, concept of
buffer, strength of buffer, range of buffer,
2 Chemical Composition of Living Systems
1. Berg Jeremy, Tymoczko John, Stryer Lubert (2001)
Biochemistry. 6th Edition, W. H. Freeman, New York.
2. Conn Eric, Stumpf Paul K., Bruuening George, Doi Roy
H., (1987) Outlines of Biochemistry. 5th Edition , John
Wieley and Sons, New Delhi.
3. Dawes Edwin A. (1972). Quantitative Problems in
Biochemistry, Churchill Livingston, Edimberg.
4. Laskin A. I. and Lechevalier H. A. (1977), CRC
Handbook of Microbiology, Vol. 1, Bacteria, CRC Press
Ohio.
5. Metzler David E. (2001) Biochemistry: The Chemical
Reactions of Living Cells, Volume 1 & 2, Academic
Press California.
6. Nelson D. L. and Cox M. M. (2002) Lehninger’s
Principles of Biochemistry, Mac Millan Worth Pub. Co.
Page 8 of 20
Course no. Title Description Total
credits
References
important biological buffers. New Delhi
MB 1.4.1 Protein Chemistry Structural features of amino acids, classification of
amino acids, amino acids as buffers, chemical
reactions of amino acids, peptide linkage: partial
double bond nature, determination of primary
structure of polypeptide (N-terminal, C-terminal
determination, method of sequencing of peptides),
structural classification of proteins, primary,
secondary, tertiary, quaternary structures of proteins.
1 7. Peberdy John F. (1980), Developmental Microbiology,
Blackie, London.
8. Segel Irvin H. (1997). Biochemical Calculations. 2nd
Ed. John Wiley and Sons, New York.
9. White Abraham, Handler Philip, Smith Emil, Hill
Rober, Lehman J. (1983) Principles of Biochemistry,
Edition 6, Tata Mc-Graw Hill Companies, Inc.
10. White David (2000) Physiology and Biochemistry of
Prokaryotes. 2nd Ed. Oxford University Press, New
York.
MB 1.5.1 Carbohydrate
Chemistry
Mono, di, oligosaccharides and polysaccharides,
with examples, asymmetric centre in sugars, Dseries,
L-series, dextro, leavo-rotatory, reducing and
non-reducing sugars, sugar anomers, sugar epimers,
sugar derivatives such as sugar alcohols, amino
sugars, sugar acids, deoxy sugars.
1
Introduction to Bioorganic Chemistry
1. Clayden, Greeves, Warren and Wothers, Organic
Chemistry, Oxford Press
Jerry March, Advanced Organic Chemistry, John Wiley
MB 1.6.1 Nucleic Acid
Chemistry
Structure of bases, nucleosides, nucleotides,
phospho-diester linkages, 5’ phosphate, 3’hydroxyl
polarity of nucleic acids, tautomeric forms of bases
and their implication in pairing of bases, structure of
DNA (A, B and Z forms), Tm value, detailed
structure of t-RNA, r-RNA, and m-RNA.
1
Page 9 of 20
Course no. Title Description Total
credits
References
MB 1.7.1 Lipid Chemistry Classification of lipids according to chemical
structure, fatty acids, saturated, unsaturated,
branched, nomenclature, system structure and
function of triglycerides, phospholipids,
sphingolipids, terpenes, prostaglandins, waxes,
steroids, detection and estimation of lipids
1
MB 1.8.1 Vitamin Chemistry Water and fat soluble vitamins: structure and
function of Thiamine, Riboflavin, Nicotinic acid,
Pantothenic acid, Pyridoxine, Biotin, Folic acid,
Lipoic acid, Glutathione, Vitamin B12, Ascorbic
acid. Structure and function of fat soluble vitamins
as vitamins A, D, E and K
1
MB 1.9.2 Biology of Yeasts
and Moulds
The vegetative cells of fungi, Growth and
development of the vegetative cell, The life cycle,
Asexual reproduction, Sexual reproduction,
Physiology of fungi, Economic importance of fungi,
Main groups of fungi
2 1. The Fungi Ed. Ainsworth , I. (1965), II (1966), III
(1968), IVA (1973), IVB (1973), Academic Press.
2. Booth, C. (1971) in Methods in Microbiology vol. 4,
ed. By J. R. Norris et al. , Academic Press, London.
3. Beech, F. W. and R. R. Davenport (1971) Isolation,
Purification and Maintenance of Yeasts, in Methods in
Microbiology vol. 4, ed. By J. R. Norris et al. ,
Academic Press, London, pp 153-182.
4. Rose, A. H. and J. S. Harrison ed. (1969) The Yeasts,
Vols. 1, 2, & 3. Academic Press, London.
MB 1.10.1 Ultra structure of
Cell Membrane
Ultrastructure and Organization of Eukaryotic
Cell
Structural organization of: Cytoskeleton (structural
proteins – microfilaments, actins, etc.); nucleus,
Mitochondria and chloroplasts and their genetic
organization, Endoplasmic Reticulum, Golgi
apparatus, Protein trafficking; Events in cell cycle,
Regulation of cell cycle
Membrane Transport:
1 Ultrastructure and Organization of Eukaryotic Cell
1. Alberts Bruce (1985) Molecular Biology of Cell.
Garland Pub.
2. Conn Eric, Stumpf Paul K., Bruuening George, Doi Roy
H., (1987) Outlines of Biochemistry Edition , John
Wieley and Sons, New Delhi.
3. De Robertis E. D. P. and De Robertis E. M. F. (1987),
Cellular and Molecular Biology Lea and Febiger,
Philadelphia.
Page 10 of 20
Course no. Title Description Total
credits
References
The composition and architecture of
membranes,Membrane dynamics, Solute transport
across membranes: Passive diffusion, active
transport using P and F type ATPases, Ion mediated
transport, transport of ions across membranes (ion
pumps), Biochemical shuttles across mitochondrial
membranes, Model membranes; Liposomes
4. Schlegel Hans G. (1995) General Microbiology, Edition
7, CUP, Cambridge.
5. Stanier R. Y., Adelberg E. A., Ingraham J. L., (1976)
General Microbiology, 4th edition, Mac Millan Press,
London.
6. Stephen W. Paddock, Confocal Microscopy, from
Methods and Protocols Vol. 122, Methods in Molecular
Biology, Humana Press, Press Inc., Totowa, NJ
Membrane Transport:
1. Nelson D. L. and Cox M. M. (2005) Lehninger’s
Principles of Biochemistry, Fourth edition, W. H.
Freeman & Co. New York.
2. Voet Donald and Voet Judith G. (1995) Biochemistry,
2nd Ed.. John Wiley and sons New York.
3. White Abraham, Handler Philip, Smith Emil, Hill
Rober, Lehman J. (1983) Principles of Biochemistry,
Edition 6, Tata Mc-Graw Hill Companies, Inc.
4. White David (2000) Physiology and Biochemistry of
Prokaryotes. 2nd Ed. Oxford University Press, New
York.
5. Zubay Geoffrey (1998) Biochemistry, 4th Ed., W. C.
Brown, New York.
6. Berg Jeremy, Tymoczko John, Stryer Lubert (2001)
Biochemistry 4th Ed, W. H. Freeman, New York.
7. Moat Albert G. and Foster John W. (1988) Microbial
Physiology 2nd Ed. John Wiley and Sons New York.
MB 1.11.1 Cell wall of
Bacteria and Fungi
Definition, important chemical features of the cell
wall, structure, function and assembly in bacteria and
archae, typical model of cell wall of bacteria and
archae, chemical differentiation of fungal cell wall
1 1. Ruiz-Herrera J, (1991) Fungal cell walls. CRC Press
ISBN:0849366720
2. Guntram Seltmann, Otto Holst (2001) Bacterial cell
walls. Springer ISBN:3540426086
MB 1.13.2 Biochemical and
Molecular Biology
Techniques
A. Principles and applications of gel filtration, ion
exchange and affinity chromatography, thin layer
and gas chromatography, high pressure liquid
chromatography (HPLC), electrophoresis and
electrofocussing, ultra centrifugation (velocity
2 Instrumentation
1. Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006)
Biochemistry. 6th Edition. Freeman, New York.
2. Cotterill, R. M. J. (2002) Biophysics: An Introduction.
John Wiley & Sons, England.
Page 11 of 20
Course no. Title Description Total
credits
References
and buoyant density
B. Principles and techniques of nucleic acid
hybridization and Cot curves; Sequencing of
Proteins and nucleic acids ; Southern, Northern
and South-Western blotting techniques ;
Polymerase chain reaction; Methods for
measuring nucleic acid & protein interactions
3. Garrett, R. H. and Grisham, C. M. (2004) Biochemistry.
3rd Ed. Brooks/Cole, Publishing Company, California.
4. Mount, D. W. (2001) Bioinformatics: sequence and
genome analysis. Cold Spring Harbor Laboratory Press,
New York.
5. Nölting, B. (2006) Methods in modern biophysics.
Second Edition. Springer, Germany.
6. Pattabhi, V. and Gautham, N. (2002) Biophysics.
Kluwer Academic Publishers, New York & Narosa
Publishing House, Delhi.
7. Wilson Keith and Walker John (2005) Principles and
Techniques of Biochemistry and Molecular Biology, 6th
Ed. Cambridge University Press, New York.
Molecular Biophysics
1. Daune M. & W. J. Duffin (1999) Molecular Biophysics:
Structures in Motion, Oxford University Press.
2. Nalting B. & B. Nalting (2003) Methods in Modern
Biophysics Springer Verlag
3. Voit E. O. (2000) Computational Analysis of
Biochemical Systems Cambridge University Press.
Other books:
1. Narayanan, P. (2000) Essentials of Biophysics. New
Age International Publication, New Delhi
2. Stephenson, F. H. (2003) Calculations in molecular
biology and biotechnology: A guide to mathematics in
the laboratory. Academic Press, Elsevier Science,
London. (For numerical problems in instrumentation)
MB 1.14.2 Biostatistics a. Quantitative methods in biology, sampling
methods, scales and variables, data organization,
tabulation, graphical representation
b. Concepts, examples and problems for each of the
following:
i. Descriptive statistics: Frequency and
2 1. Brown D. and Rothery P. 1993. Models in Ecology,
Wiley.
2. Cochran W. G. – Sampling Techniques, Wiley estern
Ltd, New Delhi.
3. Felller W. Introduction to probability theory and its
applications, Asia Publishing House, Mumbai.
Page 12 of 20
Course no. Title Description Total
credits
References
probability distributions, graphical
representation of distributions, measures of
central tendency, measures of dispersion,
skew-ness, kurtosis. Introduction to Normal,
Binomial and Poisson distributions and their
applications. Distribution of sample means,
standard error and confidence interval.
ii. Regression and correlation, curve fitting and
choice of models.
iii. Introduction to multivariate analysis: multiple
regressions, ordination, principal component
analysis.
iv. Survey design
v. Factorial design, ANOVA and F test.
vi. Probability: Laws of probability, independence
and randomness.
vii. Hypothesis testing: Comparison of two sample
means: t-tests, non-parametric tests. The
concepts of null hypothesis,
significance level, type I and type II errors, one
tailed and two tailed tests.
Categorical data and proportion data: Chi square test
and test for goodness of fit.
1. Glover T. and Mitchell K. 2002. An introduction to
Biostatistics. McGraw-Hill , N.Y.
2. Goon, Gupta and Dasgupta- Fundamentals of statistics.
World Press, Kolkota.
3. Irfan Ali Khan and Atiya Khanum, Fundamentals of
Biostatistics. 2nd Ed. Ukaaz Publications, Hyderabad.
4. Montgomery D. C. Design and analysis of experiments,
John Wiley and Sons.
5. Murthy M.N. Sampling methods, Indian Statistical
Institute, Kolkota.
6. Wayne Daniel 2007. Biostatistics, a foundation for
analysis in the health Sciences, Edn. 7, Wiley-Indian
Edn.
Theory Credits 19
M.Sc. I Sem I Practicals
MB 1.01.5 Studies on
Extremophiles
1. Isolation and characterization of anaerobic
microorganisms
2. Isolation and characterization of thermophilic
microorganisms
3. Isolation and characterization of Psychrophilic
microorganisms
4. Isolation and characterization of Alkalophilic
microorganisms
5. Isolation and characterization of Acidophilic
3
Page 13 of 20
Course no. Title Description Total
credits
References
microorganisms
6. Isolation and characterization of Halopphilic
microorganisms
MB 1.02.5 Biochemical
techniques
A) Basic Biochemistry
1. Buffer: Determination of pKa of a monoprotic
weak organic acid; Preparation of buffers using
KH2PO4 and K2HPO4, acetic acid and sodium
acetate, K2HPO4 and H3PO4
2. Colorimetry and spectrophotometry: Estimation
of sugar and total carbohydrate, estimation of
protein and nucleic acids ( DNSA, phenolsulphuric
acid method, Lowry, Bradford and
UV Spectrophotometry, Diphenyl amine and
orcinol method)
3. Determination of saponification value and iodine
number of fat
4. Chromatography: Separation of sugar and
amino acids by paper and thin layer
chromatography
5. Electrophoresis: Agarose gel electrophoresis,
PAGE and SDS-PAGE of proteins
6. Molecular weight determination by Moecular
sieve chromatography
B) Cell Disruption and separation of cell
components using Sucrose/ Ficoll Density
Gradient( yeast)
3 1. Wilson Keith and Walker John (2005) Principles and
Techniques of Biochemistry and Molecular Biology, 6th
Ed. Cambridge University Press, New York.
Practical Credits 6
Total Credits 25
Page 14 of 20
Course no. Title Description Total
credits
References
M.Sc. I Sem II Theory
MB 2.1.2 General Virology 1) Distinctive properties of viruses: size, acellular
(non cellular) organization, nature of viral
genome, etc.
2) Morphology and ultra structure of viruses:
Capsids; icosahedral, helical, envelope,
glycoprotein, matrix proteins and lipids, Viral
genome (double stranded viral DNA / RNA,
single stranded viral DNA / RNA, etc); virus
related agents (viroids, prions).
3) Cultivation of viruses: Growth of viruses in
embryonated egg, in experimental animals and
in cell cultures-primary and secondary cell lines,
suspension cell cultures and monolayer cell
cultures.
4) Assay of viruses: Physical and chemical
methods of assay, (protein, nucleic acid,
radioactivity tracers, electron microscopy, etc);
Infectivity assay of animal viruses (plaque
method, pock counting, end point method) and
infectivity assay of plant viruses.
5) Morphology and ultra-structure of
bacteriophages, one step growth curve(latent
period, Eclipse period, burst size), life cycle and
other details with special reference to T (odd and
even).
2 7. Straus J. H. and Straus E.S. (1998) Evolution of RNA
Viruses Ann. Rev. Microbiol. 42: 657 – 83
8. Luria S. E. et.al. (1978) General virology, 3rd Ed, New
York. John Wiley and Sons.
9. Fields B.N.; Knipe D. M. Chanock R.M. Hirsch M. J. (Eds)
Fields Virology, 2nd Ed. New York, Raven Press. (1996)
MB 2.2.1 Animal Virology 1) Classification and nomenclature of viruses: ICTV
recommendations
2) Life cycles and other details : DNA viruses with
special reference to herpes, pox, adeno, SV40;
RNA viruses with special reference to measles,
rabies, polio, influenza, retroviruses; Oncoviruses
and Lentiviruses (HIV).
3) Miscellaneous: Interferon, antiviral agents, slow
1 1. Stephens B. and Compons R. W. (1998) Assembly of
animal viruses at the cellular membrane Ann. Rev.
Microbiol.42:489-519
2. Reisner D. & Gross H.J. (1985) Viroids Ann. Rev.
Biochem.54:531-64
3. Prusiner S. B. (1995) The Prion Diseases, Scientific
American (1):48-57
4. Sherkar A. H. & Marion P.L. (1991) Hepo DNA viruses and
Page 15 of 20
Course no. Title Description Total
credits
References
and persistent viruses; Mechanism of virus host
interaction / Mechanism of virus persistence.
Hepatocellular Carcinomas. Ann. Rev. Microbiol.45:475-
508
5. Davis and Dulbacco Medical Microbiology
MB 2.3.1 Plant Virology 1) Classification and nomenclature.
2) Effects of viruses on plants: appearance of plants,
histology, physiology and cytology of plants.
3) Diagnostic techniques to detect viruses: In seeds,
seed stocks, and diseased plants (seed
morphology, seedling symptomatology, indicator
plants, serological methods, histochemical tests
and fluorescence microscopy.)
4) Behavior of viruses in plants: Early stages of
infection, biochemistry of virus replication,
cellular sites of virus replication and assembly
and accumulation of virus particles.
5) Transmission of plant viruses: With vectors
(insects, nematodes, fungi, etc.) without vectors
(contact, seed and pollens).
6) Prevention of crop losses due to virus infection:
virus free planting material, vector control,
disease forecasting.
7) Life cycle and other details of few plant viruses:
With special reference to TMV, Cauliflower
mosaic virus etc.
1 Gibbs Adrian & Bryan Harrison ( ) Plant Virology -The
Principles. Edward Arnold Press
MB 2.4.1 Molecular
Biology of
Bacteriophages
DNA replication in T phages, lambda phage, different
mutants in phages: rapid lysis mutants (rI, rII, rIII),
Conditional mutants e.g clear plaque mutants in l,
mechanism of packing DNA in phage heads,
Lambda- lytic cascade and lysogenic repression;
M13 replication.
1 1. Matthews R.E.F. (1985) Viral Taxonomy for the Non
virologists Ann. Rev. Microbiol. 39:451-74
MB 2.5.2 Bioenergetics Laws of thermodynamics, entropy, enthalpy, free
energy, free energy and equilibrium constant, Gibbs
free energy equation, determination of free energy of
hydrolytic and biological oxidation reduction
reactions, under standard and non-standard
2
Page 16 of 20
Course no. Title Description Total
credits
References
conditions, high energy compounds, coupled
reactions, determination of feasibility of reactions.
MB 2.6.2 Enzymology Purifications of enzyme, purification chart, kinetics of
single substrate enzyme catalyzed reaction. Kinetics
of reversible inhibitions enzyme catalyzed reactions,
King Altman approach to derive – two substrate
enzyme catalyzed reactions, types of two substrate
enzyme catalyzed reactions, concept of allosterism,
positive and negative co-operativity, models of
allosteric enzymes (Monad, Wyamann and Changuax
and Koshland, Nemethy and Filmer model), kinetics
of allosteric enzyme, Hill plot, examples of allosteric
enzymes and their significance in regulation.
2 1. Berg Jeremy, Tymoczko John, Stryer Lubert (2001)
Biochemistry 4th Ed, W. H. Freeman, New York.
2. Conn Eric, Stumpf Paul K., Bruuening George, Doi Roy H.,
(1987) Outlines of Biochemistry 5th Ed , John Wiley and
Sons, New Delhi.
3. Dawes Edwin A. (1972) Quantitative Problems in
Biochemistry, Churchill Livingston, Edinburgh.
4. Hall D. D. & Rao K. K. (1996) Photosynthesis 5th Ed.,
Cambridge University Press.
5. Mandelstam Joel and McQuillen Kenneth (1976)
Biochemistry of Bacterial Growth, Blackwell Scientific
Publication London.
6. Metzler David (2001) Biochemistry: The chemical Reactions
of Living Cells, Vol 1&2, Academic Press California.
7. Moat Albert G. & Foster John W. (1988) Microbial
Physiology 2nd Ed. John Wiley and Sons New York.
8. Nelson D. L. & Cox M. M. (2005) Lehninger’s Principles of
Biochemistry, 4th edition, W. H. Freeman & Co. NY
9. Palmer Trevor (2001) Enzymes: Biochemistry,
Biotechnology & Clinical chemistry, Horwood Pub. Co.,
England.
10. Segel Irvin H. (1997) Biochemical Calculations 2nd Ed.,
John Wiley and Sons, New York.
11. Voet Donald & Voet Judith G. (1995) Biochemistry, 2nd Ed..
John Wiley & sons New York.
12. White Abraham, Handler Philip, Smith Emil, Hill Rober,
Lehman J. (1983) Principles of Biochemistry, Edn 6, Tata
Mc-Graw Hill Companies, Inc.
13. White David (2000) Physiology and Biochemistry of
Prokaryotes. 2nd Ed. Oxford University Press, NY.
14. Zubay Geoffrey (1998) Biochemistry, 4th Ed., W.C. Brown,
New York.
Page 17 of 20
Course no. Title Description Total
credits
References
MB 2.7.1 Photosynthesis Energy consideration in photosynthesis, light and
dark reaction, electron carriers in photosynthesis,
Organization of photosystem I and II, cyclic and noncyclic
flow of electrons, Z scheme, Hill reaction,
photolysis of water. Bacterial photosynthesis: scope,
electron carriers, Photosynthetic reaction center,
cyclic flow of electrons, bacterial
photophosphorylation in various groups of
phototrophic bacteria, electron donors other than
water in anoxygenic photosynthetic bacteria.
1
MB 2.8.1 Respiration:
Aerobic,
Anaerobic
Aerobic Respiration:
Mitochondrial electron transport chain, structure and
function of ATPase (bacterial and mitochondrial),
generation and maintenance of proton motive force,
oxidative phosphorylation, inhibitors and un-couplers
of electron transport chain and oxidative
phosphorylation, Atkinson’s energy charge,
phosphorylation potential and its significance, Energy
generation in all groups of chemolithotrophs.
Anaerobic Respiration:
Concept of anaerobic respiration, oxidized sulfur
compounds, and nitrate as electron acceptor with
respect to electron transport chain and energy
generation, Biochemistry of methanogenes
1
MB 2.9.2 Environmental
Biology
A. Interactions between environment and biota ;
Concept of habitat and ecological niches;
Limiting factor ; Energy flow, food chain, food
web and tropic levels ; Ecological pyramids and
recycling, biotic community – concept, structure,
dominance, fluctuation and succession; N, P, C
and S cycles in nature.
2 1. Dash, M.C. (1993). Fundamentals of Ecology. Tata McGrw
Hill Publishing Hill Co. Ltd., New Delhi
2. Macan, T. T. (1974). Freshwater Ecology. Longman Group
Ltd., London
3. Meadows, P. S. and Campbell. (1978). An introduction to
Marine Science. Blackie and Sons Ltd., Glasgow.
4. Richards, B. N. (1987). Microbiology of Terrestrial
Page 18 of 20
Course no. Title Description Total
credits
References
B. Ecosystem dynamics and management; Stability
and complexity of ecosystems; Speciation and
extinctions; Environmental impact assessment,
Principles of conservation; Conservation
strategies; Sustainable development.
Ecosystems. Longman Scientific and Technical, N.Y.
5. Techobanoglous G. and F. L. Burton (1991). Wastewater
Engineering, Treatment, Disposal and Reuse. 3rd Ed.,
Metccalf and Eddy (Eds) Tata McGraw Hill Publishing Co.
Ltd., New Delhi.
MB 2.10.2 Mathematics for
Biology
Numbers, variables (independent, dependent and
continuous), Definition of Mathematical Function:
linear, geometric and exponential functions;
differentiation and integration of simple functions,
graphs, plots.etc.
2 Arya J.A and Lardner R.W. (1979) Mathematics for Biological
Sciences. Prentice Hall Inc.
MB 2.11.2 Systematics of
yeasts and molds
Taxonomy,( classification, nomenclature and
identification) and systematics of fungi
Classification of yeasts and Molds: Characteristics as
criteria for classification, , A system of yeast
classification, 18S rDNA nucleotide sequencing
based classification of fungi, A system of
classification of molds, Systematics of fungi
2 1. Ainsworth, G. C.(1971) Dictionary of Fungi, CMI, Surrey.
2. The Fungi Ed. Ainsworth , I. (1965), II (1966), III (1968),
IVA (1973), IVB (1973), Academic Press.
3. Barnett, J. A. , R. W. Payne, and D. Yarrow (1979) A guide
to identifying and classifying yeasts, Cambridge Univ. Press,
Cambridge.
4. Campbell, I. (1974) Methods of Numerical Taxonomy for
various genra of Yeasts, in Advances in Applied Microbiology,
ed. By D. Perlman, A.P., N.Y., 17: 135-156.
5. Hawksworth, D. L. (1974). Mycologist’s Handbook, CMI
MB 2.12.1 Bioinformatics
1. Databases
Biological resource databases – Examples and
application – sequence Analysis – protein and
nucleic acid.
2. Genomics and proteomics
Sequencing genomes – sequence assembly –
genome on the web – annotating and analyzing
genome sequences. proteomics – biochemical
pathway databases.
3. Sequence analysis
Pair wise sequence comparison. protein data
bank, Swiss-prot, Genebank – sequence queries
1 1. Baldi, P. and Brunak, S. (2001) Bioinformatics: The
machine learning approach. Bradford Book, MIT Press,
Cambridge.
2. Baxevanis, A. D. and Ouellette, B. F. F. (2001)
Bioinformatics: A practical guide to the analysis of genes
and proteins. 2nd Edition. John Wiley & Sons, New York.
3. Ewens Warren J. and Gregory R. Grant. (2004) Statistical
Methods in Bioinformatics, An Introduction, Springer, New
York.
4. Lacroix, Z. and Critchlow, T. (Eds.) 2003. Bioinformatics.
Managing Scientific Data. Morgan Kaufmann Publishers.
5. Misener, S. and Krawetz, S. A. (Eds.). 2000. Methods in
Molecular Biology, Volume 132. Bioinformatics: Methods
Page 19 of 20
Course no. Title Description Total
credits
References
against biological databases – BLAST and
FASTA – multifunctional tools for sequence
analysis. multiple sequence alignments,
phylogenetic alignment – profiles and motifs.
& Protocols. Humana Press, New Jersey.
6. Mount, D. W. (2001) Bioinformatics: sequence and genome
analysis. Cold Spring Harbor Laboratory Press, NY.
7. Zoe L. & Terence C. (2004) Bioinformatics: Managing
Scientific Data, Morgan Kaufmann Publishers, New Delhi.
MB 2.13.1 Biophysical
techniques
Principles of biophysical methods used for analysis of
biopolymer structure, X-ray diffraction, fluorescence,
UV, GRO, CO, Visible, NMR and ESR spectroscopy
; Hydrodynamic methods, Atomic absorption and
plasma emission spectroscopy.
1 Instrumentation
1. Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006)
Biochemistry. 6th Edition. Freeman, New York.
2. Cotterill, R. M. J. (2002) Biophysics: An Introduction. John
Wiley & Sons, England.
3. Drenth, J. (2007) Principles of protein X-ray
crystallography. 3rd Ed. Springer, Germany.
4. Garrett, R. H. and Grisham, C. M. (2004) Biochemistry.
3rd Ed. Brooks/Cole, Publishing Company, California.
5. Keeler, J. (2002) Understanding NMR Spectroscopy. John
Wiley & Sons, England.
6. Mount, D. W. (2001) Bioinformatics: sequence and
genome analysis. Cold Spring Harbor Laboratory Press,
New York.
7. Nölting, B. (2006) Methods in modern biophysics. Second
Edition. Springer, Germany.
8. Pattabhi, V. and Gautham, N. (2002) Biophysics. Kluwer
Academic Publishers, New York and Narosa Publishing
House, Delhi.
9. Wilson Keith and Walker John (2005) Principles and
Techniques of Biochemistry and Molecular Biology, 6th Ed.
Cambridge University Press, New York.
MB 2.14.1 Radioisotopes in
Biology
Principles and applications of tracer techniques in
biology ; Radiation dosimetry ; Radioactive isotopes
and half life of isotopes ; Effect of radiation on
biological system ; Autoradiography ; Cerenkoy
radiation ; Liquid spectrometry.
1 Molecular Biophysics
1. Cavanagh John et.al. (1995) Proteins NMR Spectroscopy:
Principles and Practice, Academic Press
2. Daune M. & W. J. Duffin (1999) Molecular Biophysics:
Structures in Motion, Oxford University Press.
3. Nalting B. & B. Nalting (2003) Methods in Modern
Biophysics Springer Verlag
Page 20 of 20
Course no. Title Description Total
credits
References
4. Voit E. O. (2000) Computational Analysis of Biochemical
Systems Cambridge University Press.
Other books:
1. Narayanan, P. (2000) Essentials of Biophysics. New Age
International Publication, New Delhi
Stephenson, F. H. (2003) Calculations in molecular biology and
biotechnology: A guide to mathematics in the laboratory.
Academic Press, Elsevier Science, London. (For numerical
problems in instrumentation)
Theory Credits 22
M.Sc. I Sem II Practicals
MB 2.01.5 Experimental
Enzymology
1. Purification of enzyme from natural source
2. Determination of Km and Vm values of
enzyme :e.g.Invertase,amylase
3
MB 2.02.5 Ecology and
Environmental
Biology
1. Isolation, of cellulose degraders
2. Isolation of chitinase degraders
3. Isolation of pesticide degraders.
4. Isolation of Aflatoxin producing organism
5. Detection of Aflatoxin in food / culture
3
Practical Credits 6
Total Credits 26
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